Tris-Cl (Starvation Buffer)

The following recipe makes a stock solution of 1 M. Step 3 outlines how to make working concetration of Tris-Cl (commonly used as Starvation Buffer for Tetrahymena).

Materials

 * Tris base
 * Concentrated HCl
 * ddH2O

Method (makes 1 L of media):

 * 1) Dissolve 121.6 Tris base in 800 ml of ddH2O. Adjust the pH to the desired value by adding concentrated HCl (gradually or drop-wise).
 * 2) Adjust volume to 1 L and sterilize by autoclaving.
 * 3) To make working concentration of Tris (used as Starvation Buffer), add 10 mL of stock 1 M solution to 990 mL of ddH2O. H2O can be autoclaved prior to addition of stock solution.