NKC Solution

NKC solution can also be used to starve Tetrahymena.

Working Solution: NaCl 0.2%, KCl 0.008%, CaCl2 0.012%

Materials

 * NaCl
 * KCl
 * CaCl2

Method (makes 1 L of buffer)

 * 1) Measure out 2 g of NaCl in a liter bottle.
 * 2) Add 80 mg of KCl and 120 mg of CaCl2 to the liter bottle.
 * 3) Sterilize the buffer by autoclaving.