NDS+Pronase

NDS+Pronase is used for Whole Genome DNA Extraction.

Materials

 * SDS (20%)
 * Tris-Cl (1 M, pH 8.0)
 * EDTA (0.5 M)
 * Pronase (20 mg/mL)

Method (makes 3 mL of lysis buffer)
Add Pronase to NDS solution just prior to use in DNA extraction.
 * 1) Measure out 100 uL of 20% SDS in a 15 mL conical tube.
 * 2) Add 20 uL of 1 M Tris-Cl.
 * 3) Add 2.78 mL of 0.5 M EDTA.
 * 4) Add 100 uL of Pronase to a final volume of 3 mL.

''Addition of EDTA to SDS may cause it to precipitate. Incubate NDS solution at 55 C for a few minutes before adding Pronase solution as this will allow SDS to go back into solution.''