SOC

This recipe makes 1 L of medium.

Glucose should be added only after the autoclaved solution has been sufficiently cooled ~50-55  ° C. Sterilized solution of glucose is prepared by passing it through a 0.2 µm filter.

SOC medium can be stored at room temperature in small aliquots and is stable for several years  at -20 °C.

Method

 * 1) Fill flask 3/4 volume of flask with ddH2O.
 * 2) Then add the tryptone, yeast extract, NaCl and KCl and mix. Once the solids have dissolved bring up the volume to 1 L.
 * 3) Sterilize using the LIQUID cycle.
 * 4) Allow to cool for an hour and then add MgCl2 and sterile solution of glucose.
 * 5) Mix again by stirring.
 * 6) Pour medium into bottles by working very close to a flame to ensure sterile transfer.  Label bottles and store.