4X SDS Loading Buffer (for proteins)

Materials

 * Tris-Cl (200 mM, pH 6.8)
 * DTT (400 mM)
 * SDS ( 8%)
 * Bromophenol Blue ( 0.4%)
 * Glycerol ( 40%)
 * ddH2O

Method (makes 50 mL)

 * 1) Add 20 mL glycerol to a Falcon tube. Add glycerol slowly with a pipette to ensure proper volume is dispensed.
 * 2) Add 10 mL of Tris-Cl (1 M, pH 6.8).
 * 3) Measure 3.08 g of DTT and 4 g of SDS and add these to the tube.
 * 4) Measure 200 mg of bromophenol blue dye and add to tube.
 * 5) Bring up the volume to 50 mL with ddH2O and shake gently for 30 minutes to allow components to dissolve. Decant SDS Loading Buffer in new 50 mL tube. Avoid agitation as that will result in foam formation.