NEFF

Modified NEFF medium is slightly less richer medium than SPP.

Working Concentration: Proteose Peptone 0.25%, Yeast Extract 0.25%, Glucose 0.55%, FeCl3 33 uM 

Materials

 * Proteose peptone
 * Yeast Extract
 * Glucose
 * FeCl3-6H2O (1 M)

Method makes 1 L of media

 * 1) Measure out 2.5 g of proteose peptone in a beaker.
 * 2) Add 2.5 g of yeast extract to the flask.
 * 3) Add 5.5 g of glucose to the flask.
 * 4) Add ddH2O to final volume of 1 L.
 * 5) Add 0.02 g of FeCl3-6H2O to the flask.
 * 6) Sterilize the medium by autoclaving.