Tris Buffered Saline (TBS)

1XTBS is most commonly used in our lab for FLAG affinity purification.

Working Concentration: 50 mM Tris.HCl, pH 7.5 and 150 mM NaCl.

Materials

 * ddH2O


 * Tris.HCl (1 M, pH 7.4)


 * NaCl (5 M)

Method (makes 1 L of solution)

 * 1) Measure out 920 mL of ddH2O in a bottle.
 * 2) Add 50 mL of Tris.Cl 1 M, pH 7.4 and then 30 mL of NaCl 5 M. Mix well and sterilize by autoclaving.