Protocols

All the protocols used in our lab.

Tetrahymena Protocols
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 * Biolistic Transformation of Tetrahymena
 * Culturing Tetrahymena
 * Starving and Mating Tetrahymena
 * Counting Tetrahymena with a Hemacytometer
 * DNA Damage Test
 * Flow Cytometry to measure DNA content in Tetrahymena
 * Fluorescence Microscopy of Tetrahymena
 * Isolating Tetrahymena in Drop Medium
 * Isolation and Purification of Tetrahymena nuclei
 * Long-Term Storage of Tetrahymena
 * ​Thawing Tetrahymena cells
 * Phenotypic Assortment to reduce Gene Copy Number
 * Short-term Storage of Tetrahymena
 * Whole Cell Extract of Tetrahymena cells
 * Whole Genome DNA extraction from Tetrahymena
 * Whole Genome DNA extraction from Tetrahymena (short protocol)
 * TBD

Bacterial Protocols

 * Glycerol Stocks of E. Coli
 * Transformation of E. Coli

Biochemical Protocols

 * Bradford Assay to determine Protein Concentration
 * DNA Precipitation of Restriction Digests
 * Ethanol Precipitation of DNA
 * LR Reaction using LR Clonase II
 * Preparation of protein samples for Mass Spectrometry
 * Recharging Ni Resin
 * SDS-PAGE
 * TCA Precipitation of Proteins
 * Western Blotting