Dithiothreitol (DTT)

''Lyophilized DTT powder is hygroscopic and should be stored desiccated at 4 C with a stability of several years. DTT is not stable in solution. A freshly made DTT solution aliquot should be made prior to each use.''

Materials
''This product is soluble in water (up to 50 mg/mL), yielding a clear, colorless solution. DTT is also soluble in ethanol and acetone.''
 * Dithiothreitol
 * ddH2O

Method

 * 1) Measure out 0.385 g of DTT into a 15 mL conical tube.
 * 2) Add 5 mL sterile ddH 2 O to the tube.
 * 3) Aliquot into sterile microcentrifuge tubes and freeze at -20 C.