Short-term Storage of Tetrahymena

Materials

 * SPP or NEFF medium
 * Glass tubes (with twist-off caps)
 * 250 μg/ml Penicillin G & Streptomycin sulfate
 * Amphotericin (fungizome)
 * Selective antibiotics
 * Mineral Oil

Method

 * 1) Pipette 10 mL of SPP or NEFF medium in sterile glass tubes. Add selective antibiotics as needed.
 * 2) Add 1-5 uL of Tetrahymena cultures into growth medium.
 * 3) Overlay with 1 mL of mineral oil.   Use large orifice pipette tips for pipetting and draw and dispense the oil slowly. 
 * 4) Place the tubes in drawer at room temperature.   Cell cultures will stay good for 1-2 month. Transfer to fresh media accordingly.