Ethylenediaminetetraacetic acid (EDTA)

Materials

 * Disodium EDTA-2H2O
 * NaOH
 * ddH2O

Method (makes 1 L of solution)

 * 1) Fill a bottle with 800 mL of ddH2O.
 * 2) Add 186.1 g of disodium EDTA-2H2O to the bottle and stir vigorously on a magnetic stir plate.
 * 3) Adjust the pH to 8.0 with NaOH.  Disodium EDTA•2H2O will not go into solution until the pH is adjusted ~8.0 by the addition of NaOH. 
 * 4) Bring the volume up to 1 L with ddH2O.
 * 5) Dispense into aliquots and sterilize by autoclaving.