LB

This recipe makes 1 L of LB medium used for culturing E. coli.

LB medium should be stored at room temperature and is stable for 1 year in these conditions.

Method

 * 1) Fill 3/4 volume of the flask with ddH2O.
 * 2) Add a magnetic stir bar, and stir while adding all the dry ingredients.
 * 3) Adjust the pH to 7.5. Most likely you will have to use 1 N HCl.
 * 4) Adjust the volume to 1 L with ddH2O.
 * 5) Sterilize by autoclaving using the LIQUID cycle.